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Sample Lab Report
August 01, 2025

Sample Lab Report

 

The Impact of Temperature Variations on Silkworm Growth Rate and Cocoon Production

 

1- Objectives

The main object of this report is to find out how different temperature conditions affect the overall growth, molting and cocoon formation of the silkworm larvae.

2- Introduction

Silkworms (Bombyx mori) are basically cold-blooded insects whose metabolic rates primarily depend on environmental temperatures. Optimal temperatures (23–26°C) accelerate growth whereas extreme conditions affect their development adversely. This study is an attempt: to examine, via experiment, how controlled temperatures influence larva growth speed and silk yield; and to determine what ideal temperatures are best for silkworm’s metabolism.

3- Material and Apparatus

  1. 15 healthy silkworm larvae (of 3rd instar)
  2. Fresh mulberry leaves
  3. Thermometers
  4. Digital scale (.01g precision)
  5. 3 temperature-controlled environments:

  • Group A: (Room temperature)
  • Group B: (Heated incubator)
  • Group C: (Cool cabinet)

4- Procedure

A total of 15 larvae were collected and divided into 3 groups (5 in each container). They were supplied with equal amount of fresh mulberry leaves at the same time each day. The learners recorded the following data of worms on daily basis:

  • Length (from head to tail)
  • Mass (weight of each entire group)
  • Molting events (skin shedding turns)
  • Cocoon formation (counted days)

The experiment continued until all larvae were transformed into cocoons. Finally, total cocoons of each group were collected separately and weighed to reach final conclusions.

5- Data Analysis

Table 1: Average Larval Growth (Day 1–10)

Temp. Group Avg. Length (cm) Avg. Mass (g) Days to Molt Days to Cocoon
25°C (Control) 2.8 → 6.5 1.2 → 8.7 Day 4 Day 22
30°C (Warm) 2.8 → 7.1 1.2 → 9.3 Day 3 Day 18
20°C (Cool) 2.8 → 4.9 1.2 → 6.1 Day 6 Day 28

Table 2: Cocoon Yield

Group Avg. Cocoon Weight (g) Silk Quality
25°C 1.45 Shiny, firm
30°C 1.20 Fragile, uneven
20°C 1.60 Dense, but smaller

Discussion

Growth Speed:

The group of larvae put in warm conditions (30°C) grew fastest reached 7.1 cm in length but produced weaker and lighter in weight cocoons. On the other hand, the larvae kept at (20°C) recorded slowest growth but developed into denser cocoons.

Molting:

The warmer were the temperatures, the faster was molting.

Cocoon Quality:

The larvae kept at (25°C) turned into the best quality shiny and firm cocoons whereas those fostered at (30°C) produced uneven and brittle silk of lowest quality.

Conclusion

The study at lab has proved that temperature variations impact silkworm development significantly. A cross in optimal temperature limits (23–26°C) may result in the production of bad quality silk.

Precautions

  1. Handle larvae gently with soft brushes
  2. Replace mulberry leaves every 12 hours
  3. Clean containers daily to avoid mold

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